Plasma Membrane Protein Extraction Kit from Abcam

Product Specs
ItemPlasma Membrane Protein Extraction Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65400
Quantity50 test
Time1h 0m
TargetPlasma Membrane protein extraction

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Targeting endoplasmic reticulum associated degradation pathway combined with radiotherapy enhances the immunogenicity of esophageal cancer cellsCancer Biology & TherapyDecember 31, 2023Hui Luo, Yanan Sun, Liuxiang Wang, Hui Liu, Ran Zhao, Mengqiu Song, Hong Gewere isolated from the total membrane component by using the plasma membrane protein extraction kit (ab65400, Abcam, UK). Cell surface CLAR and sodium-potassium ATPase (Na+/K+ ATPase) were detected in the western blot

(2) Receptor-interacting protein 1 and 3 kinase activity are required for high-fat diet induced liver injury in miceFront Endocrinol (Lausanne)December 15, 2023Xiaoqin Wu, Rakesh K. Arya, Emily Huang, Megan R. McMullen, Laura E. Nagyfrom murine liver Plasma fractions were isolated using the plasma membrane protein extraction kit (ab65400, Abcam). Liver tissues were resuspended in homogenization buffer and lysed using a Dounce homogenizer

(3) Targeting protein methylation in pancreatic cancer cells results in KRAS signaling imbalance and inhibition of autophagyCell Death & DiseaseNovember 23, 2023María F. Montenegro, Román Martí-Díaz, Ana Navarro, Jorge Tolivia, Luis Sánchez-del-Campo, Juan Cabezas-Herrera, José Neptuno Rodríguez-LópezCell fractionation The plasma membrane fraction was isolated using a plasma membrane protein extraction kit (Abcam; ab65400) following the manufacturer’s instructions. In brief, cells were lysed in homogenization buffer containing

(4) Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscleNature CommunicationsAugust 17, 2023Anastasia Sacharidou, Ken Chambliss, Jun Peng, Jose Barrera, Keiji Tanigaki, Katherine Luby-Phelps, İpek Özdemir, Sohaib Khan, Shashank R. Sirsi, Sung Hoon Kim, et al.were isolated from total cellular membranes by phase separation (Abcam plasma membrane extraction kit; ab65400). Following treatment, the cells were washed with ice cold PBS and pelleted twice, and then homogenized

(5) An insulin-regulated arrestin domain protein controls hepatic glucagon actionThe Journal of biological chemistryAugust 1, 2023Dagdeviren S, Hoang MF, Sarikhani M, Meier V, Benoit JC, Okawa MC, Melnik VY, Ricci-Blair EM, Foot N, Friedline RH, et al.Plasma Membrane isolation kit from Abcam that removes all cellular membrane proteins (catalog no.: ab65400). For quantitative RT–PCR, RNA was extracted from homogenized liver samples by the TRIzol (Life Sciences)

(6) MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFRCancer communications (London, England)July 1, 2023Luo L, Wei D, Pan Y, Wang QX, Feng JX, Yu B, Kang T, Luo J, Yang J, Gao S.experiments. Plasma membrane proteins were isolated using the Plasma Membrane Protein Extraction Kit (ab65400, Abcam). Briefly, cells were harvested, washed twice with ice‐cold PBS, and resuspended in homogenization

(7) ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell CarcinomaCancer researchJune 15, 2023Sun Y, Zhu L, Liu P, Zhang H, Guo F, Jin X.intracellular membrane (ICM) fractions were isolated with the Plasma Membrane Protein Extraction Kit (Abcam, ab65400). Briefly, cells were incubated with 2 mL of homogenized buffer at 4°C after being washed three times

(8) Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivoAutophagyApril 1, 2023Wu SY, Wu HT, Wang YC, Chang CJ, Shan YS, Wu SR, Chiu YC, Hsu CL, Juan HF, Lan KY, et al.independent mice. Plasma membrane protein extraction A plasma membrane protein extraction kit (Abcam, ab65400) was used to purify the cytosol and plasma membrane fraction from MIN6 cells after treatment of LG or

(9) Defective BVES-mediated feedback control of cAMP in muscular dystrophyNature communicationsMarch 30, 2023Li H, Wang P, Zhang C, Zuo Y, Zhou Y, Han R.(Bio-Rad, Hercules, CA). The membrane extraction was performed using the Membrane Protein Extraction Kit (ab65400, Abcam, Waltham, MA) according to the manufacturer’s instruction. The extracted protein samples were

(10) Transglutaminase 2 associated with PI3K and PTEN in a membrane-bound signalosome platform blunts cell deathCell Death & DiseaseMarch 28, 2023Károly Jambrovics, Pál Botó, Attila Pap, Zsolt Sarang, Zsuzsanna Kolostyák, Zsolt Czimmerer, Istvan Szatmari, László Fésüs, Iván P. Uray, Zoltán Balajthy[12]. Plasma membrane preparation The plasma membrane was isolated according to the Abcam protocol (ab65400). PIP3 extraction and measurement The isolation of PIP molecules was carried out by chloroform-methanol

(11) Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2Nature communicationsFebruary 16, 2023Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams LA, Stolz A, Lichtenthaler SF, Elazar Z, Behrends C.subsequently processed as described above (proximity labeling). PM enrichment An PM extraction kit (Abcam, Ab65400) was used according to the manufacturer’s guidelines. Cells were collected by scaping, homogenized in

(12) Inhibition of sphingosine-1-phosphate receptor 3 suppresses ATP-induced NLRP3 inflammasome activation in macrophages via TWIK2-mediated potassium effluxFrontiers in immunologyJanuary 1, 2023Wang Y, Wang C, He Q, Chen G, Yu J, Cang J, Zhong M.protein separation The plasma membrane was isolated using Plasma Membrane Protein Extraction Kit (ab65400, Abcam, UK) according to the manufacturer’s instructions. One 10-cm plate of cells for each condition

(13) Overexpression of Rhodopsin or Its Mutants Leads to Energy Metabolism Dysfunction in 661w CellsInvestigative Opthalmology & Visual ScienceDecember 5, 2022Yang Liu, Xin Wang, Ruowen Gong, Gezhi Xu, Min Zhuand plasma membrane proteins were extracted using an Abcam Plasma Membrane Protein Extraction Kit (ab65400). Cells were harvested, homogenized, and centrifuged at 700g for 10 minutes. The supernatants were centrifuged

(14) Geranylgeranyl pyrophosphate depletion by statins compromises skeletal muscle insulin sensitivityJournal of Cachexia, Sarcopenia and MuscleDecember 1, 2022Lai Wang, Zuguo Zheng, Lijun Zhu, Lingchang Meng, Hanling Liu, Keke Wang, Jun Chen, Ping Li, Hua Yanginformation. Isolation of plasma membrane fractionation The Plasma Membrane Protein Extraction Kit (ab65400, Abcam) was used to isolate plasma membrane proteins from C2C12 myotubes and primary mouse myotubes

(15) HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinomaNature metabolismOctober 1, 2022Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ, et al.from Sigma. PalmB (178581) was purchased from Millipore. The Plasma Membrane Protein Extraction Kit (ab65400) was purchased from Abcam. GoTaq qPCR Master Mix (A6001) was purchased from Promega. Sirius red (G1018)

(16) An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memoryNature cell biologyOctober 1, 2022Lanna A, Vaz B, D'Ambra C, Valvo S, Vuotto C, Chiurchiù V, Devine O, Sanchez M, Borsellino G, Akbar AN, et al.telomere vesicles, T cell plasma membranes were then purified with Plasma Membrane Protein Extraction Kit (ab65400; Abcam) and absence APC telomeres assessed by confocal imaging on Leica SP2. Plasma membrane purification

(17) Ppt1-deficiency dysregulates lysosomal Ca⁺⁺ homeostasis contributing to pathogenesis in a mouse model of CLN1 diseaseJournal of inherited metabolic diseaseMay 1, 2022Mondal A, Appu AP, Sadhukhan T, Bagh MB, Previde RM, Sadhukhan S, Stojilkovic S, Liu A, Mukherjee AB.isolation The plasma membrane fractions were isolated using Plasma Membrane Protein Extraction Kit (Abcam, Cat#ab65400) according to the manufacturer’s protocol. Western blot analysis Western blot analyses were performed

(18) Pulmonary endothelial NEDD9 and the prothrombotic pathophenotype of acute respiratory distress syndrome due to SARS-CoV-2 infectionPulmonary circulationApril 1, 2022Alba GA, Samokhin AO, Wang RS, Wertheim BM, Haley KJ, Padera RF, Vargas SO, Rosas IO, Hariri LP, Shih A, et al.isolated plasma membrane fractions of HPAEC lysates using a Plasma Membrane Protein Extraction Kit (Abcam, ab65400) according to the manufacturer's instructions. Magnetic beads (Bio‐Rad Protein G SureBeads) were resuspended

(19) AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degenerationCommunications biologyDecember 9, 2021Miyagishima KJ, Sharma R, Nimmagadda M, Clore-Gronenborn K, Qureshy Z, Ortolan D, Bose D, Farnoodian M, Zhang C, Fausey A, et al.Plasma membrane preparation of iRPE were prepared using a Plasma Membrane Protein Extraction Kit (Abcam, #ab65400) following the manufacturer’s recommendations. In brief, 9 million iRPE cells were collected from transwells

(20) High glucose induces trafficking of prorenin receptor and stimulates profibrotic factors in the collecting ductScientific ReportsDecember 1, 2021Venkateswara R. Gogulamudi, Danielle Y. Arita, Camille R. T. Bourgeois, Justine Jorgensen, Jing He, William C. Wimley, Ryosuke Satou, Alexis A. Gonzalez, Minolfa C. Prietosupplement serum free media for 1, 6 h specified in each protocol. Abcam Plasma Membrane Isolation Kit (Ab65400; ABCAM, Cambridge) was used to extract the membrane proteins. Western blotting was performed using antibody

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Plasma Membrane Protein Extraction Kit from Abcam

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Plasma Membrane Protein Extraction Kit from Abcam

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